The aim of the present study was to evaluate protein carbonyl concentration as a predictor of AKI development in patients with septic shock and of renal replacement therapy (RRT) and mortality in patients with SAKI. with SAKI. Among 66 patients with SAKI, 77% died during the ICU stay. Protein carbonyl concentration was not associated with RRT in patients with SAKI. However, the ROC curve analysis revealed that higher levels of protein carbonyl were associated with mortality in these patients. In logistic regression models, protein carbonyl level was associated with SAKI development (OR: 1.416; 95% CI: 1.247C1.609; for 10 min). The pellets were washed three times with ethanol-ethyl acetate (1:1; vol./vol.) mixture and resuspended in 6 mol/l guanidine hydrochloride at 37C for 10 min. The level of protein carbonyl was quantified spectrophotometrically at 360 nm using an extinction coefficient 22,000 M?1 cm?1 Laboratorial analysis Hemograms were performed with a Coulter STKS hematologic autoanalyzer (Luton/Bedfordshire, U.K.). Total serum levels of C-reactive protein (CRP), albumin, glycemia, creatinine, and urea were measured using the dry chemistry method (Ortho-Clinical Diagnostics VITROS 950?, Johnson & Johnson), and lactate was measured using a Roche OMNI? S Blood Gas Analyzer. Statistical evaluation Data are expressed as the mean SD or the median (like the lower and higher quartiles). Statistical comparisons between groupings for constant variables had been performed using the Learners = 0.235; em P /em =0.007). Furthermore, proteins carbonyl concentrations had been higher in sufferers who created SAKI (Desk 1). The AUC of proteins carbonyl focus was 0.621, with a 95% CI of 0.523C0.719 (Figure 2). Open up in another window Figure 1 Movement diagram of studied sufferers Open in another window Figure 2 Receiver working curves evaluation(A) ROC curve for the association between proteins carbonyl concentrations and sepsis-AKI advancement in 129 sufferers with septic shock (AUC: 0.621; 95% CI: 0.523C0.719; em P /em =0.018). (B) ROC curve for the association between proteins carbonyl concentrations buy ZM-447439 and sepsis-AKI mortality in 66 sufferers (AUC: 0.958; 95% CI: 0.892C1.000; em P /em 0.001). Table 1 Demographic, scientific, and laboratory data of 129 sufferers with septic shock thead th align=”left” rowspan=”1″ colspan=”1″ Variables /th th colspan=”2″ align=”middle” rowspan=”1″ Sepsis-induced AKI advancement /th th align=”center” rowspan=”1″ colspan=”1″ em P /em -worth /th th align=”left” rowspan=”1″ colspan=”1″ /th th align=”middle” rowspan=”1″ colspan=”1″ buy ZM-447439 Yes ( em n /em =66) /th th align=”center” rowspan=”1″ colspan=”1″ No ( em n /em =63) /th th align=”still left” rowspan=”1″ colspan=”1″ /th /thead Age group (years)67.0 (58.3C75.3)64.0 (51.0C73.0)0.22Male, em n /em (%)32 (48.5)29 (46.0)0.92APACHE II score18.8 6.016.1 6.50.01SOFA score10.0 (8.0C12.0)8.0 (7.0C10.0) 0.001RBC transfusions, em n /em (%)35 (53.0)28 (44.4)0.42Sepsis focus, em n /em (%)0.45??Respiratory41 (62.1)32 (50.8)??Abdominal16 (24.2)16 (25.4)??Urinary3 (4.6)4 (6.3)??Others6 (9.1)11 (17.5)RRT, em n /em (%)12 (18.2)0 (0)0.001CKD, em n /em (%)57 (86.4)7 (11.1) 0.001ICU mortality, em n /em (%)51 (77.3)35 (55.6)0.015Lactate (mmol/l)2.2 (1.4C3.6)2.1 (1.2C3.5)0.79Hemoglobin (g/dl)11.0 (9.1C12.0)11.6 (10.0C12.7)0.04Hematocrit (%)32.1 6.534.0 5.60.08Leukocytes (103/mm3)16.7 (12.2C21.8)16.3 (12.2C23.6)0.86Glycemia (mg/dl)145 (118C186)145 (115C190)0.90CRP (mg/dl)35.9 (27.7C44.2)31.5 (8.4C39.6)0.04Proteins carbonyl (nmol/ml)27.8 (15.9C33.1)19.4 (12.2C29.1)0.018Albumin (g/dl)2.2 (2.0C2.5)2.1 (1.8C2.8)0.56Urea (mg/dl)97 (68C159)53 (32C88) 0.001Creatinine (mg/dl)2.1 (1.6C2.7)0.7 (0.5C1.1) 0.001 Open up in another window Abbreviations: APACHE II, Acute Physiology and Chronic Wellness Evaluation; CKD, chronic kidney disease; CRP, C-reactive proteins; ICU, Intensive Treatment Device; MV, mechanical ventilation; RBC, red bloodstream cellular material; RRT, renal substitute therapy; SOFA, Sequential Organ Failure Evaluation. Data are expressed as a mean SD, median (like the lower and higher quartiles), or percentage. The demographic and scientific data of the 66 sufferers with SAKI are shown in Desk 2. Among these sufferers, 77% passed away during ICU stay, 48% were man, and the median proteins carbonyl concentrations had been 27.8 (15.9C33.1) nmol/ml. Just the APACHE II and SOFA ratings had been higher in sufferers who passed away during ICU stay. Upon evaluation of the laboratory data, just higher degrees of proteins carbonyl were connected with mortality in SAKI (Table 2). However, protein carbonyl focus was not connected with RRT in sufferers with SAKI [RRT ( em n /em =12): 30.8 (24.1C35.1) nmol/ml; non-RRT ( em n /em =54): 27.1 (13.6C33.1) nmol/ml; em P /em =0.25]. Desk 2 Demographic, clinical, and laboratory data of 66 patients with septic shock who developed acute kidney injury during ICU stay thead th align=”left” rowspan=”1″ colspan=”1″ Variables buy ZM-447439 /th th colspan=”2″ align=”center” rowspan=”1″ ICU mortality /th th align=”center” rowspan=”1″ colspan=”1″ em P /em -value /th th align=”left” rowspan=”1″ colspan=”1″ /th th align=”center” rowspan=”1″ colspan=”1″ Yes ( em n /em =51) /th th align=”center” rowspan=”1″ colspan=”1″ No ( em n /em =15) /th th align=”left” rowspan=”1″ colspan=”1″ /th /thead Age (years)67.0 (60.0C76.0)64.0 (51.0C75.0)0.40Male, em n /em (%)27 (52.9)5 (33.3)0.30APACHE II score20.0 5.914.8 4.70.003SOFA score10.7 2.68.6 1.70.005RBC transfusions, em n /em (%)28 (54.9)7 (46.7)0.79Sepsis focus, em n /em (%)0.46??Respiratory32 (62.7)9 Rabbit Polyclonal to PAK7 (60.0)??Abdominal11 (21.6)5 (33.3)??Urinary2 (3.9)1 (6.7)??Others6 (11.8)0 (0)RRT, em n /em (%)11 (21.6)1 (6.7)0.27CKD, em n /em (%)43 (84.3)14 (93.3)0.67Lactate, (mmol/l)2.2 (1.3C3.4)2.4 (1.5C4.5)0.83Hemoglobin, (g/dl)10.9 (9.1C11.8)11.2 (9.0C12.3)0.65Hematocrit (%)32.1 6.432.1 7.00.99Leukocytes (103/mm3)16.6 (12.1C23.9)17.0 (12.2C19.4)0.62Glycemia (mg/dl)138 (116C182)153 (132C186)0.20CRP (mg/dl)36.2 (26.7C44.1)35.5 (30.2C44.3)0.59Protein carbonyl (nmol/ml)29.5 6.8011.3 6.32 0.001Albumin (g/dl)2.2.